Articles

  • Dr. med. Christian Pohlkamp
    from 21.04.2021
    New panel for clarifying unclear erythrocytosis/polyglobulia

    Polyglobulia or erythrocytosis without detection of one of the typical mutations, such as the JAK2 mutation in polycythemia vera, poses problems for us hematologists. New scientific findings open up further options in molecular genetic clarification.

    Post review
    Read article
  • Dr. med. Adriane Koppelle
    from 20.04.2021
    Advances in the targeted therapy of lymphoma

    The therapeutic landscape for lymphoma diseases has changed considerably in recent years due to our progress in understanding their pathogenesis. A major advantage has been to reduce the need for chemotherapy and to be able to use new types of targeted therapies alone or in combination. In this article we report in particular on BCL2 inhibition and its resistance mechanisms, as well as the role of the MYD88 mutation as well as the CXCR4 mutation in Waldenström’s disease.

    Post review
    Read article
  • Prof. Dr. med. Dr. phil. Torsten Haferlach
    from 24.02.2021
    62. ASH Annual Meeting & Exposition - a follow-up report

    In December 2020, the 62nd Annual Meeting & Exposition of the American Society of Hematology (ASH) took place, for the first time in virtual format. At the world's largest hematology congress, international experts gathered for five days to exchange the latest hematology findings and learn about the current state of research. This time, too, the MLL Munich Leukemia Laboratory was there. A total of 12 scientists presented their current research projects. You can find out which ones in this article.

    Post review
    Read article
  • PD Dr. med. Gregor Hörmann, PhD
    from 16.12.2020
    Molecular genetics in mastocytosis and hypereosinophilia

    Advanced molecular genetic testing is gaining ground in the diagnosis of mast cell diseases and hypereosinophilia. In addition to the typical mastocytosis-driver mutation KIT D816 as well as PDGFRA, PDGFRB, FGFR1, and PCM1-JAK2 rearrangements, the MLL uses advanced Next-Generation Sequencing (NGS) mutation analysis to inform the diagnosis and prognostic stratification of these diseases. These NGS gene panels are continuously updated to reflect the latest advances in scientific understanding.

    Post review
    Read article
  • Dr. med. Adriane Koppelle
    from 16.12.2020
    Advances in the targeted therapy of hematological neoplasm

    In recent years, research into the underlying pathogenetic mechanisms of hematologic neoplasms has led to remarkable advances in our understanding of these diseases. Cytogenetic and molecular aberrations are the most important factors in determining response to chemotherapy and are increasingly also therapeutic targets. We would first like to provide an overview of novel therapies for AML whose target genes can be sequenced in MLL.

    Post review
    Read article
  • Dr. rer. nat. Ines Schmidts
    from 30.09.2020
    MDS-Associated Aberrant Phenotypes in Multiple Myeloma

    It has been known for about a decade that in some patients with multiple myeloma (MM), MDS-associated changes in the bone marrow can be detected at the time of diagnosis or later during the course of disease. These include genetic alterations, such as evidence of clonal hematopoiesis and/or MDS-associated cytogenetic anomalies*, as well as aberrant immunophenotypes typical of MDS **. A recent study underlines the clinical relevance of such MDS-associated (immuno)phenotypic anomalies (MDS-PAs) and indicates

    Post review
    Read article
  • Dr. Wencke Walter
    from 18.08.2020
    Diagnostic perspectives – WGS and WTS

    The diagnosis and classification of hematological neoplasia is a complex process. Various diagnostic technologies (cytomorphology, histology, cytogenetics, immunophenotyping and molecular genetics) are needed to meet the clinical challenges for a rapid, accurate and therapeutically relevant diagnosis for each patient. Genetic testing in particular has changed considerably in recent years, making it a quick and comprehensive option for a variety of issues.

    Post review
    Read article
  • Dr. med. Christian Pohlkamp
    from 08.06.2020
    Identifying resistance mutations in targeted therapies

    Over the past few years, the use of targeted therapies has proven effective for various forms of hematological neoplasia, and increasingly become routine. The rational molecular design of such active substances allows for the targeted blocking of intracellular signal chains in malignant cells, resulting e.g. in cell cycle arrest or ideally apoptosis. Unfortunately, resistance occurs even against such tailored therapies.

    Post review
    Read article
  • Dr. rer. nat. Constance Bär
    from 18.02.2020
    Assessing Sequence Variants

    Sequencing technologies have developed rapidly in the last few years. It is possible to sequence an entire genome or a larger panel within just a few days. At the MLL, data from the sequencing machine is loaded to our private cloud in real time and automatically preprocessed using the very latest algorithms.

    Post review
    Read article
  • from 16.11.2019
    Standardization of the BCR-ABL1 Quantification in CML

    When using molecular tests to quantify BCR-ABL1 in CML patients, it is recommended that the test be performed in a standardized laboratory. Standardization ensures that results comply with the ELN (European LeukemiaNet; Baccarani et al., Blood 2013) guidelines, enables comparisons to be made between laboratories and serves as quality assurance.

    Post review
    Read article
  • from 05.11.2019
    Immunophenotyping in multiple myeloma: Patients without minimal residual disease live longer

    The prognostic significance of minimal residual disease (MRD) in patients with multiple myeloma has been examined in numerous studies and is the subject of much controversial discussion. Existing data has to date been extremely heterogeneous with regard to patient collectives, therapies, number of cases, and the methodology via which MRD was detected.

    Post review
    Read article
  • from 01.11.2019
    Additional molecular genetic diagnostics for multiple myeloma

    Approx. 10% of all hematological neoplasias are plasma cell diseases such as multiple myeloma (MM) or plasma cell leukemias. As is the case for other hematological diseases, molecular genetic diagnostics can be viewed as increasingly relevant in the diagnosis, prognosis and therapeutic stratification of MM.

    Post review
    Read article
  • Dr. med. Christian Pohlkamp
    from 30.10.2019
    Munich Leukemia Laboratory MLL offers integrated findings for myelodysplastic syndromes

    After the successful introduction of Integrated Findings for acute myeloid leukemia (AML) at the end of 2017, Münchner Leukämielabor GmbH (MLL) now also offers a version for myelodysplastic syndromes (MDS). The goal here is also to provide both a clear summary and detailed interpretation of the often complex constellation of individual cytomorphological, flow cytometry, and in particular cytogenetic and molecular genetic findings.

    Post review
    Read article
  • Dr. med. Christian Pohlkamp
    from 28.10.2019
    Step-by-step diagnosis at MLL

    Cost-conscious diagnostics are a must. As MLL hematologists who, in general, have invariably spent a lot of time working in hospitals and outpatient clinics, we are particularly aware of this problem. It has thus always been our priority to provide you and your patients with a cost-effective procedure in the form of step-by-step diagnostics.

    Post review
    Read article
  • Dr. rer. nat. Ines Schmidts
    from 23.10.2019
    The importance of advanced molecular diagnostics in cytopenia and (suspected) MDS

    Next-generation sequencing (NGS) has rapidly become an indispensable tool in diagnostic molecular genetics. A recent article in Blood (Steensma Blood 2018) examined how patients with ambiguous cytopenia or with a (suspected) MDS diagnosis can benefit from the diagnostic potential of NGS.

    Post review
    Read article
  • Dr. rer. nat. Ines Schmidts
    from 17.10.2019
    The importance of next-generation sequencing (NGS) diagnostics in (suspected) acute myeloid leukemia (AML)

    In the last issue (1/2019), MLL News featured an article which was recently published in Blood on the use of high-throughput sequencing in (unclear) cytopenia and MDS (Steensma et al. 2018). Next-generation sequencing (NGS) is already playing a major role in AML diagnostics today and will continue to become more and more important.

    Post review
    Read article