Immunophenotyping

Characterization and quantification of benign and malignant cell populations in peripheral blood or bone marrow aspirate and determination of minimal/measurable residual disease (MRD).

Testing material
  • Bone marrow
  • Peripheral blood
Processing time

2 hours to 1 day

Test material

Between 5 and 10 ml of peripheral blood or bone marrow aspirate with heparin as anticoagulant are needed for immunophenotyping. Alternatively, EDTA can also be used as anticoagulant, although it is associated with a faster loss of cell viability. Peripheral blood is sufficient as a test material, provided that malignant cells have infiltrated peripheral blood. Therefore, bone marrow aspirate should be tested if peripheral blood is not infiltrated.

Methodology

Characterization involves multi-parametric flow cytometry (MFC) of the analyzed cell populations based on their light-scattering properties. This procedure uses fluorescence-marked monoclonal antibodies that are targeted at diagnostically relevant antigens on the cell membrane and in the cytoplasm. Modern flow cytometers enable simultaneous detection of several different fluorochromes and hence allow a precise description of antigen expression patterns for approximately 1,000 cells per second. This means that even cell populations with a frequency of 1% or less can be characterized very quickly.

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Request form

Please enclose this completed examination form with your sample shipment. The form represents all diagnostic methods and analyses completely.

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Request form hemoglobinopathy

Order for laboratory and molecular genetic testing for hemoglobinopathies.

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Patient consent - Research projects

By providing this patient consent, you agree to the use of excess biomaterial for research purposes.

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Primary sample collection manual

Detailed information on test material and shipment can be found in our primary sample collection manual.

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Information for correct sampling of buccal swap and nail material

Here you will find information on the correct sampling of germline material.

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FAQs - questions and answers

If you have questions about placing an order, diagnostics or reports, you will find the right answers in our FAQs on the Service site.

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Your contact person

»We achieve higher quality through interdisciplinarity.«

Prof. Dr. med. Wolfgang Kern

Executive Management
Internist, Hematologist and Oncologist
Deputy Head of Immunophenotyping

T: +49 89 99017-200